Analysis of protein-ligand interactions by fluorescence polarization
نویسندگان
چکیده
منابع مشابه
Quantitative Analysis of Multiple Antibody-ligand Interactions in a Microchip Using Fluorescence Polarization Anisotropy
We present a microfluidic device that allows quantitative analysis of protein interactions with fluorescently labeled ligands. Dissociation constants (Kd), binding classes (m) as well as number of binding sites (n) can be determined in the same experiment, even if protein mixtures are present.
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We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea. We find that the fluorescence becomes six times brighter when the excitation, or emission, beam is polarized parallel (compared with perpendicular) to the crystal long a...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2011
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2011.305